Volume 3, Issue 1, March 2019, Page: 24-29
Selection of Entomopathogenic Nematodes to Control Nymphs of Haplaxius crudus (Van Duzee) (Hemiptera: Cixiidae)
Miriam Rosero Guerrero, Colombian Oil Palm Research Center, Paratebueno, Colombia
Alex Enrique Bustillo Pardey, Colombian Oil Palm Research Center, Bogotá, Colombia
Received: Jan. 29, 2019;       Accepted: Mar. 20, 2019;       Published: May 15, 2019
DOI: 10.11648/j.aje.20190301.14      View  48      Downloads  10
Abstract
Haplaxius crudus transmits the pathogen that causes Lethal wilt (ML for its name in Spanish), one of the main diseases that affects oil palms in Colombia. In its nymphal stage it feeds from grasses present at the plantations, and adults feed on oil palm leaves. With the objective of controlling the nymphal stage of H. crudus, the effectiveness of the following entomopathogenic nematodes was assessed: Steinernema colombiense, S. websteri, Steinernema sp. 1, Steinernema sp. 2, Heterorhabditis bacteriophora, Heterorhabditis sp. (Gua 31), Heterorhabditis sp. (Gua 236), Heterorhabditis sp. (CPHsp1301) and Heterorhabditis sp. (CPHsp1302). Pathogenicity was assessed under laboratory conditions using Petri dishes with roots of Paspalum virgatum. Virulence was assessed using mesh houses with PVC tubes and plastic trays with P. virgatum. Once the most virulent nematode was selected, three dosage levels were assessed under simulated field conditions, in order to select the most effective dosage. All assessed nematode species were pathogenic to nymphs of H. crudus, and nymph stage IV was the most susceptible stage, with death rates of over 80%. Regarding virulence, there were statistically significant differences among treatments (P≤0.05), producing nematode death rates between 28.3 and 88.2%. Heterorhabditis sp. (CPHsp1301), obtained from the soil of palm plantations, was selected because it caused a mortality rate of 78.4% with at a dosage of 1300 IJ/cm2 in the sprayed area. The results are promising and further research should be performed under commercial oil palm plantation conditions.
Keywords
Oil Palm, Biological Control, Steinernema, Heterorhabditis
To cite this article
Miriam Rosero Guerrero, Alex Enrique Bustillo Pardey, Selection of Entomopathogenic Nematodes to Control Nymphs of Haplaxius crudus (Van Duzee) (Hemiptera: Cixiidae), American Journal of Entomology. Vol. 3, No. 1, 2019, pp. 24-29. doi: 10.11648/j.aje.20190301.14
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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