The worldwide demand for industrially essential enzymes is growing due to their eco-friendly and sustainable applications in broad industrial sectors. The main objective of this investigation is intended to isolate potent alkalophilic native bacteria from local habitats for the cost-effective production of an enzyme alkaline protease. For screening of alkalophilic bacteria, bacterial samples were taken from various natural alkaline habitats, and bacterial cultures were screened on agar plate having skimmed milk as protein substrate by using the protein hydrolysis method. The bacteria showing maximum proteolytic potential were identified by microscopic, biochemical, and 16S rDNA analysis. Additionally, the culture components and other medium parameters have been optimized for higher enzyme production. High yield alkaline protease was obtained with conditions of 1% inoculum, pH 9.0, and at 37°C temperature, with 1% sugarcane molasses as the best suitable carbon substrate. Partial pur¬ification of the enzyme was performed and characterized for their optimum activity on various parameters (temperatures and pH range). The approx. molecular weight of the enzyme was estimated to be ~35 kDa. This study demonstrates the production of an industrially important enzyme (alkaline protease) from a newly isolated native Bacillus spp. Economical production of this enzyme can be commercially applied in vast industrial sectors like agriculture, textile, food, detergent, and leather industries.
| Published in | International Journal of Microbiology and Biotechnology (Volume 6, Issue 4) |
| DOI | 10.11648/j.ijmb.20210604.15 |
| Page(s) | 137-146 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Agro-industrial Residues, Alkaline Protease, Bacillus Spp, Cost-effective, Sugarcane Molasses
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APA Style
Ranjeet Singh, Ravindra Meena, Harsh Kumar, Banwari Lal. (2021). Production, Purification, and Characterization of an Industrially Important Enzyme Alkaline Protease Produced from Locally Isolated Bacillus Bacteria. International Journal of Microbiology and Biotechnology, 6(4), 137-146. https://doi.org/10.11648/j.ijmb.20210604.15
ACS Style
Ranjeet Singh; Ravindra Meena; Harsh Kumar; Banwari Lal. Production, Purification, and Characterization of an Industrially Important Enzyme Alkaline Protease Produced from Locally Isolated Bacillus Bacteria. Int. J. Microbiol. Biotechnol. 2021, 6(4), 137-146. doi: 10.11648/j.ijmb.20210604.15
AMA Style
Ranjeet Singh, Ravindra Meena, Harsh Kumar, Banwari Lal. Production, Purification, and Characterization of an Industrially Important Enzyme Alkaline Protease Produced from Locally Isolated Bacillus Bacteria. Int J Microbiol Biotechnol. 2021;6(4):137-146. doi: 10.11648/j.ijmb.20210604.15
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Download@article{10.11648/j.ijmb.20210604.15,
author = {Ranjeet Singh and Ravindra Meena and Harsh Kumar and Banwari Lal},
title = {Production, Purification, and Characterization of an Industrially Important Enzyme Alkaline Protease Produced from Locally Isolated Bacillus Bacteria},
journal = {International Journal of Microbiology and Biotechnology},
volume = {6},
number = {4},
pages = {137-146},
doi = {10.11648/j.ijmb.20210604.15},
url = {https://doi.org/10.11648/j.ijmb.20210604.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20210604.15},
abstract = {The worldwide demand for industrially essential enzymes is growing due to their eco-friendly and sustainable applications in broad industrial sectors. The main objective of this investigation is intended to isolate potent alkalophilic native bacteria from local habitats for the cost-effective production of an enzyme alkaline protease. For screening of alkalophilic bacteria, bacterial samples were taken from various natural alkaline habitats, and bacterial cultures were screened on agar plate having skimmed milk as protein substrate by using the protein hydrolysis method. The bacteria showing maximum proteolytic potential were identified by microscopic, biochemical, and 16S rDNA analysis. Additionally, the culture components and other medium parameters have been optimized for higher enzyme production. High yield alkaline protease was obtained with conditions of 1% inoculum, pH 9.0, and at 37°C temperature, with 1% sugarcane molasses as the best suitable carbon substrate. Partial pur¬ification of the enzyme was performed and characterized for their optimum activity on various parameters (temperatures and pH range). The approx. molecular weight of the enzyme was estimated to be ~35 kDa. This study demonstrates the production of an industrially important enzyme (alkaline protease) from a newly isolated native Bacillus spp. Economical production of this enzyme can be commercially applied in vast industrial sectors like agriculture, textile, food, detergent, and leather industries.},
year = {2021}
}
TY - JOUR T1 - Production, Purification, and Characterization of an Industrially Important Enzyme Alkaline Protease Produced from Locally Isolated Bacillus Bacteria AU - Ranjeet Singh AU - Ravindra Meena AU - Harsh Kumar AU - Banwari Lal Y1 - 2021/12/24 PY - 2021 N1 - https://doi.org/10.11648/j.ijmb.20210604.15 DO - 10.11648/j.ijmb.20210604.15 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 137 EP - 146 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20210604.15 AB - The worldwide demand for industrially essential enzymes is growing due to their eco-friendly and sustainable applications in broad industrial sectors. The main objective of this investigation is intended to isolate potent alkalophilic native bacteria from local habitats for the cost-effective production of an enzyme alkaline protease. For screening of alkalophilic bacteria, bacterial samples were taken from various natural alkaline habitats, and bacterial cultures were screened on agar plate having skimmed milk as protein substrate by using the protein hydrolysis method. The bacteria showing maximum proteolytic potential were identified by microscopic, biochemical, and 16S rDNA analysis. Additionally, the culture components and other medium parameters have been optimized for higher enzyme production. High yield alkaline protease was obtained with conditions of 1% inoculum, pH 9.0, and at 37°C temperature, with 1% sugarcane molasses as the best suitable carbon substrate. Partial pur¬ification of the enzyme was performed and characterized for their optimum activity on various parameters (temperatures and pH range). The approx. molecular weight of the enzyme was estimated to be ~35 kDa. This study demonstrates the production of an industrially important enzyme (alkaline protease) from a newly isolated native Bacillus spp. Economical production of this enzyme can be commercially applied in vast industrial sectors like agriculture, textile, food, detergent, and leather industries. VL - 6 IS - 4 ER -
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